EFFECT OF IRRADIANCE SPECTRA ON THE PHOTOINDUCED TOXICITY OF THREE POLYCYCLIC AROMAfIC HYDROCARBONS

摘要

Photoinduced toxicity of polycyclic aromatic hydrocarbons (PAHs) is dependent on the concentration of compounds present and the dose of light received. Of the light present, only those wavelengths absorbed by the compound have the potential to initiate the photochemical events underlying phototoxicity. This suggests that variation in light spectra present in natural waters, arising from variation in dissolved organic carbon composition, is an important determinant of phototoxicity risk in specific, PAH- contaminated waterbodies. To quantify the effect of environmentally realistic variation in light spectra on toxicity, brine shrimp (Artemia salina) assays were conducted under various light spectra and with three PAHs (pyrene, fluoranthene, and anthracene) of known phototoxicity potential. In these spectral assays, the total ultraviolet light present was equivalent; only the spectral char- acteristics varied. Based on the absorbance spectra of these PAHs, it was predicted that toxicity, quantified using immobilization as the endpoint, would vary significantly among light spectra in pyrene assays, but not in anthracene assays, and that variation in toxicity in fluoranthene assays would be intermediate. The results supported these assumptions. In the pyrene exposures, the glass filter time to 50 population immobilization (IT50) (39.5 min) was ll7 longer than the KCr filter IT50 (l8.2 min). In the fluoranthene exposures, the glass filter IT50 (49.5 min) was 27 Ionger than the KCr filter IT50 (39.l min). In the anthracene exposures, the glass filter IT50 (62.2 min) was not st