STRUCTURE-SPECIFICITY RELATIONSHIPS FOR HALOALKANE DEHALOGENASES

摘要

A structural analysis of the substrate specificity of hydrolytic dehalogenases originating from three differant bacterial isolates has been performed using the multiple computer-automated structure evaluation methodology. This methodology identifies structural fragments in substrate molecules that either activate or deactivate biological processes. The analysis presented in this contribution is based on newly measured dehalogenation data combined with data from the literature (91 substrates). The enzymes under study represent different specificity classes of haloalkane dehalogenases (haloaikane dehalogenase from Xanthobacter au- totrophicus GJ10, Rhodococcus erythropolis Y2, and Sphingomonas paucimobilis UT26).