QUANTIFICATION OF METALLOTHIONEIN ISOFORMS IN FISH LIVER AND ITS IMPLICATIONS FOR BIOMONITORING

摘要

A rapid, reproducible, and sensitive ion-exchange chromatography(IEC)method combined with graphite-furnace atomic absorption spectrometry(GF-AAS)was developed to separate and quantify basal of both metallothionein(MT)isoforms in dab(Limanda limanda)liver samples. Dab liver homogenates were saturated with Cd, and obtained cytosols were purified by a two-step acetone precipitation prior to chromatographic analysis. Metallothionein isoforms were separated by IEC and were subsequently quantified indirectly by GF-ASS via their Cd contents.