A novel integrated bio-reactor of moving bed and constructed wetland (MBCW) for domestic wastewater treatment and its microbial community diversity

摘要

An MBBR and CW combo bio-reactor (MBCW) was designed as a novel hybrid process for simultaneous organic, nitrogen and phosphate removal through the long-term operation. The effect of the internal recycling rate (IRR), hydraulic retention time (HRT) and chemical oxygen demand/total nitrogen (C/N) ratio were all discussed, and the recommended values were 5:1, 12 h and 6, respectively. A higher C/N ratio was a key factor for achieving a higher TN removal. The mixed biocarrier system was realized by inoculating porous polymer carriers (PPC) and cylindrical polyethylene carriers (CPC) and achieving a higher organic biodegradation and nitrification rate compared to a single carrier system. Microorganism activities and plants' uptake or utilization both contributed to the nutrient removal in a constructed wetland. High-throughput sequencing results revealed an abundant microbial diversity and a distinct microbial distribution in the whole system where Flavobacterium (14.2%), Acinetobacter (12.87%) and Rhodobacter (10.83%) dominated on PPC, Terrimonas (8.88%), Reyranella (6.61%) and Rubinisphaera (5.63%) dominated on CPC, Comamonas (4.18%), Gemmobacter (4.02%) and Hydrogenophaga (3.97%) dominated on CWs, as well as Citrobacter (53.13%) on suspended floc.