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首页> 外文期刊>Journal of Virology >Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cells.
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Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cells.

机译:棉尾兔乳头瘤病毒早期地区的转录及其在COS-7细胞中鉴定两种E6多肽。

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摘要

Cottontail rabbit papillomavirus (CRPV) early proteins are present at very low levels in virus-induced tumors and cannot be detected by immunological methods. Furthermore, cells in culture are not readily transformed by the virus. To overcome these difficulties in identifying and characterizing the putative transforming protein(s) coded by the E6 open reading frame, the early cottontail rabbit papillomavirus region was expressed under the control of the late simian virus 40 promoter. Mapping of the transcripts in transiently transfected COS-7 cells indicated that transcription was initiated in the late region of simian virus 40. Two E6-coded polypeptides were identified, representing translation products initiated at the first and second AUG codons.
机译:棉尾兔乳头瘤病毒(CRPV)早期蛋白质存在于病毒诱导的肿瘤中的非常低的水平,不能通过免疫方法检测。此外,培养物中的细胞不容易被病毒转化。为了克服这些困难,在鉴定和表征由E6开放阅读框架编码的推定转化蛋白,早期的棉尾兔乳头瘤病毒区在晚期硅藻土40启动子的控制下表达。瞬时转染的COS-7细胞的转录物的映射表明,转录在Simian病毒40的后期开始。鉴定出两种E6编码的多肽,代表在第一和第二八个八个八个八个八个八个八个八码子上发起的翻译产品。

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