Selective Amplification of Exons 3 and 8 of the Human Growth Hormone Receptor (hGHR) Gene Based on Newly identified Intron Sequences

摘要

The gene for human growth hormone receptor (hGHR) consists of at least 10 exons, and the cor- responding protein is encoded in exons- 2-10 which span at least 87 kbp of chromosome 5. Failure to amplify exons 3-and 8 of the hGhr gene from Japanese subjects with the previously reported primers prompted us to determine intron sequences flanking exon 3 and those flanking exon 8 of the hGHR gene, and noel intron sequences flanking exons 3 and 8 of the hGHR gene were identified.