Development of Element Technology for 1 STEP Biomarker Protein Analysis Device Using Silver Nanoparticle-Contained Hydrogel and Reagent-Immobilized Cartridge

摘要

We fabricated 1 STEP biomarker protein analysis device using silver nanoparticles (AgNPs)-contained hydrogel and reagent-immobilized cartridge. Specific color change of AgNPs based on localized surface plasmon resonance (LSPR) was applied to develop the novel colorimetric protein analysis device. In this report, we constructed this device by four components, AgNPs-contained hydrogel, glucose oxidase (GOx) antibody release pad, glucose release pad, and antibody-immobilized membrane. AgNPs-contained hydrogel detected 0.001 to 10 μg/mL GOx-antibody. AgNPs-contained hydrogel absorbance became altered 90.0% GOx release pad as compared to GOx-antibody solution under the reaction time 60 min. AgNPs-contained hydrogel absorbance became altered 98.4% glucose release pad as compared to glucose solution under the reaction time 60 min. We applied AgNPs-contained hydrogel and antibody-immobilized membrane to detect human immunoglobulin G (IgG). As a result, we found these could detect 1 μg/mL or lower concentration of human IgG.