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Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons

机译:喂食多环芳烃混合物的鲑鱼气单胞菌攻击的虹鳟鱼头部肾脏先天免疫基因的转录变化

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摘要

We previously observed that exposure to a complex mixture of high molecular weight polycyclic aromatic hydrocarbons (PAHs) increased sensitivity of rainbow trout (Oncorhynchus mykiss) to subsequent challenge with Aeromonas salmonicida, the causative agent of furunculosis. In this study, we evaluate potential mechanisms associated with disease susceptibility from combined environmental factors of dietary PAH exposure and pathogen challenge. Rainbow trout were fed a mixture of ten high molecular weight PAHs at an environmentally relevant concentration (7.82 mu g PAH mixture/g fish/day) or control diet for 50 days. After 50 days of PAH exposure, fish were challenged with either Aeromonas salmonicida at a lethal concentration 30 (LC30) or growth media without the pathogen (mock challenge). Head kidneys were collected 2, 4, 10 and 20 days after challenge and gene expression (q < 0.05) was evaluated among treatments. In animals fed the PAH contaminated diet, we observed down-regulation of expression for innate immune system genes in pathways (p < 0.05) for the terminal steps of the complement cascade (complement component C6) and other bacteriolytic processes (lysozyme type II) potentially underlying increased disease susceptibility after pathogen challenge. Increased expression of genes associated with hemorrhage/tissue remodeling/inflammation pathways (p < 0.05) was likely related to more severe head kidney damage due to infection in PAH-fed compared to control-fed fish. This study is the first to evaluate transcriptional signatures associated with the impact of chronic exposure to an environmentally relevant mixture of PAHs in disease susceptibility and immunity.
机译:我们以前观察到,暴露于高分子量多环芳烃(PAHs)的复杂混合物中会增加虹鳟(Oncorhynchus mykiss)对随后感染沙门氏菌(Aurmonas salicida)(糠fur病的病原体)的敏感性。在这项研究中,我们从饮食中PAH暴露和病原体攻击的综合环境因素评估与疾病易感性相关的潜在机制。给虹鳟鱼喂食十种高分子量PAHs的混合物(环境相关浓度)(7.82μgPAH混合物/ g鱼/天)或对照饮食50天。 PAH暴露50天后,用致死浓度30(LC30)的鲑鱼气单胞菌或无病原体的生长培养基对鱼进行攻击(模拟攻击)。在攻击后第2、4、10和20天收集头肾脏,并评估治疗之间的基因表达(q <0.05)。在受PAH污染的饮食喂养的动物中,我们观察到补体级联反应(补体成分C6)的最终步骤和其他细菌分解过程(II型溶菌酶)最终途径的先天免疫系统基因表达下调(p <0.05)病原体攻击后潜在的疾病易感性增加。与出血/组织重塑/炎症途径相关的基因表达的增加(p <0.05),可能与PAH喂养的鱼因感染PAH而导致的更严重的头部肾脏损害有关。这项研究是第一个评估与慢性暴露于环境相关的PAHs混合物对疾病易感性和免疫力的影响相关的转录特征的研究。

著录项

  • 来源
    《Ecotoxicology and Environmental Safety》 |2017年第8期|157-163|共7页
  • 作者单位

    Oregon State Univ, Dept Environm & Mol Toxicol, ALS 1007, Corvallis, OR 97331 USA;

    Oregon State Univ, Dept Environm & Mol Toxicol, ALS 1007, Corvallis, OR 97331 USA;

    Oregon State Univ, Dept Integrat & Comparat Biol, Cordley 3029, Corvallis, OR 97331 USA;

    Oregon State Univ, Dept Environm & Mol Toxicol, ALS 1007, Corvallis, OR 97331 USA;

    Natl Marine Fisheries Serv, Environm & Fisheries Sci Div, Northwest Fisheries Sci Ctr, NOAA, 2030 South Marine Sci Dr, Newport, OR 97365 USA;

    Univ Calif Davis, Dept Civil & Environm Engn, 1 Shields Ave, Davis, CA 95616 USA;

    Univ Calif Davis, Dept Civil & Environm Engn, 1 Shields Ave, Davis, CA 95616 USA;

    Natl Marine Fisheries Serv, Environm & Fisheries Sci Div, Northwest Fisheries Sci Ctr, NOAA, 2727 Montlake Blvd East, Seattle, WA 98112 USA;

    Natl Marine Fisheries Serv, Environm & Fisheries Sci Div, Northwest Fisheries Sci Ctr, NOAA, 2727 Montlake Blvd East, Seattle, WA 98112 USA;

    Oregon State Univ, Dept Environm & Mol Toxicol, ALS 1007, Corvallis, OR 97331 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Disease susceptibility; Polycyclic aromatic hydrocarbon mixture; Innate immunity; Transcriptomics. Rainbow trout;

    机译:疾病易感性;多环芳烃混合物;先天免疫性;转录组学虹鳟鱼;

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