Electron Microscopic Investigation of Nitrobenzene Distribution and Effect on Plant Root Tip Cells Ultrastructure

摘要

Electron microscopic radioautography demonstrated the pen- etration of [1-6~(14)C]nitrobenzene in maize and soybean root tip cells: radioactive label was detected in cell wall, plasmalemma, nuclei, and cytoplasm. Among cytoplasmic organelles, the high- est label was found in mitochondria and plastids. [1-6~(14)C]nit- robenzene and/or products of its transformation accumulated in vacuoles. Study of the action of different concentrations of nit- robenzene on cell ultrastructural organization revealed the fol- lowing picture. Nitrobenzene concentration up to 0.015 mM was harmless for plant cells. Increase of nitrobenzene concentration from 0.015 to 1.5 mM induced several pathological changes, up to the complete destruction of cells.