Analysis of the Effect of the Polyprenol Preparation Ropren and the Choline Alphoscerate Preparation Gliatilin on the Membrane-Bound and Soluble Forms of Cholinesterases and Monoamine Oxidase of Rat Brain and Serum in the Tetrachloromethane Model System o

摘要

With regard for an increased interest to designing new therapeutic and prophylactic tools of plant origin containing a broad spectrum of biologically active compounds, broadening the spectrum of their physiological efficacy is a relevant problem. One of such preparations, isolated from the neutral part of needles of pine and spruce, is ropren-a representative of the group of polyprenols (long-chain polyisoprene alcohols). It was demonstrated earlier that ropren exhibits immunomodulatory properties and stimulates humoral response of the organism to phagocytosis, which may be successfully used for treating various infectious, allergic, and autoimmune diseases. In addition, these are grounds to assume that ropren displays certain cerebroprotective and hepatoprotective effects. For this reason, it was of interest to use the hepatotropic model of hepatic encephalopathy induced by injecting animals with tetrachloromethane (carbon tetrachloride, CCl_4) for comparing the effects of the polyprenol preparation ropren and the choline alphoscerate preparation gliatilin, which has an integrated effect on neuronal function. Such a comparison may be a key stage of targeted screening of physiological efficacy of biologically active polyprenol compounds. However, when the tetrachloromethane modelrnused, it should be taken into account that tetrachloromethane is a strong hepatotoxic agent, mostly due to a pronounced membranotropic effect. It can be easily understood how a hydrophobic solvent, such as CCl_4, can affect the lipid components of the membrane . For this reason, the problem of establishment of the role of biologically active preparations in physiological processes may also be related to analysis of their membranotropic effect. The method that most adequately corresponds to the goals of this study is enzymatic analysis, due to its versatility and high sensitivity.