Reconstitution of Gluxotoxic HIT-T15 Cells With Somatostatin Transcription Factor-1 Partially Restores Insulin Promoter Activity

摘要

We have reported that chronic culture of HIT-T15 cells inmedium containing supraphysiologic glucose con- centrations (11.1 mmol/l) causes a decrease in insulin mRNA levels, insulin content, and insulin release. Fur- thermore, decrease in insulin gene transcription and binding activity of two essential β-cell transcription factor, somatostatin transcription factor-1 (SFT-1; also known as GSTF, IDX-1, IPF-1, PDX-1, and GSF) and RIPE-3b1 activator, are associated with this glucotoxic Effect.