Rapid Publication: Hormone-Sensitive Lipase, the Rate-Limiting Enzyme in Triglyceride Hydrolysis, Is Expressed And Active in β -Cells

摘要

Triglycerides in the β-cell may be important for stim- ulus-secretion coupling, through provision of a lipid- derived signal, and for pathogenetic events in NIDDM, where lipids may adversely affect β-cell function. In adipose tissues, hormone-sensitive lipase (HSL) is rate-limiting in triglyceride hydrolysis. Here, we investigated whether this enzyme is also expressed and active in β-cells. Northern blot analysis and reverse transcription-polymerase chain reaction demonstrated that HSL is expressed in rat islets and in the clonal β-cells lines INS-1, RINm5F, and HIT-T15.