Glucose-Induced [Ca~2+]_i Abnormalities in Human Pancreatic Islets

摘要

Chronic hyperglycemia desensitizes β-cells to glucose. To further define the mechanisms behind desenitiza- Tion and the role of overstimulation, we tested human Pancreatic islets for the effects of long-term elevated Glucose levels on cytoplasmic free Ca~2+ concentration ([Ca~2+]_i) and its relationship to voerstimulation. Islets were cultured for 48 h with 5.5 or 27 mmol/l glucose.