Rapid Publication: ATP~4-Mediates Closure of Pancreatic β-Cell ATP-Sensitive Potassium Channels by Interaction With 1 of 4 Identical Sties

摘要

In pancreatic β-cells, cytosoli [ATP~4-] critically controls insulin secretion via inhibition of ATP-sensitive potas- sium (K_ATP) channels. These channels are heteromulti- mers composed with a 4:4 stoichiometry of an inwardly rectifying K~+ channel subunit (Kir6.2) ;lus a regulatory sulfonylurea receptor. To elucidate stoichiometry of ATP~4- action, we analyzed ATP~4- sensitivity of channels Coassembled form wild-type Kir6.2 and a loss of ATP~4- Sensitivity mutant (G334D).