Endothelin 1 Transcription Is Controlled by Nuclear Factor-kB in AGE-Stimulated Cultured Endothelial Cells

摘要

Incubation of bovine aortic endothelial cells (BAECs) with erythrocytes from patients with type 2 diabetes induced an increase in endothelin 1 (ET-1) production. The effect of erythrocytes on ET-1 synthesis was Dependent on glycemic control. ET-1 levels after incu- Bation with erythrocytes derived from patients with HbA_1c levels <6/100 were just half the levels observed After incubation with erythrocytes from patients with HbA_lc levels >8/100. N~ε-(carboxymethyl)lysine (CML)- Containing protein isolated form patients' erythrocytes Induced ET-1, and CML-containing protein-dependent ET-1 induction was blocked by the recombinant decoy Peptide soluble receptor for advaned glycation end Products (AGEs), which comprises the NH_2-terminal Ig Domain of the receptor for AGEs.