Identification of genes involved in glucose-stimulated insulin secretion

摘要

Type 1 and type 2 diabetes are widespread diseases, and the numbers of afflicted individuals are increasing. It is now well recognized that it is very difficult to con- trol blood glucose adequately by insulin injection ther- apy. Our laboratory has been investigating the poten- tial utility of genetically engineered cell lines for insulin replacement therapy. One aspect of this initia- tive is to define all of the genes that control glucose- stimulated insulin secretion(GSIS). Although a variety of rodent insulinoma cell lines have been described, they lines are often not suitable for genetic studies because they exhibit either very small or left-shifted glucose responses relative to normal islets.