Human fructosamine-3-kinase: purification, sequencing, substrate specificity, and evidence of activity in vivo

摘要

Nonenzymatic glycation appears to be an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fruc- Tosamines, such as protein-bound fructoselysines. In This report, we describe the purification and character- Ization of a mammalian fructosamine-3-kinase(FN3K) Which phosphorylates fructoselysine(FL)residues on Glycated proteins, to FL-3-phosphate(FL3P). This phos- Phorylation destabilizes the FL adduct and leads to its Spontaneous decomposition, thereby reversing the non- Enzymatic glycation process at an early stage.