C_2-ceramide influences the expression and insulin-mediated regulation of cyclic nucleotide phosphodiesterase 3B and lipolysis in 3T3-L1 adipocytes

摘要

Cyclic nucleotide phosphodiesterase(PDE)3B plays an important role in the antilipolytic action of insulin and, thereby, the release of fatty acids from adipocytes. Increased concentrations of circulating fatty acids as a Result of elevated or unrestrained lipolysis cause insu- Lin resistance. The lipolytic action of tumor necrosis Factor(TNF)-αis though to be one of the mechanisms By which TNF-αinduces insulin resistance. Ceramide is The suggested second messenger of TNF-αaction, and in This study, we used 3T3-L1 adipocytes to investigate the Effects of C_2-ceramide(a short-chain ceramide analog) On the expression and regulation of PDE3B and lipo- Lysis.