Antifungal activity of methanolic leaf extract of Carthamus oxycantha against Rhizoctonia solani

摘要

Potato is a globally important crop whose production is severely damaged by black scurf disease (stem cankers and tuber blemishes) caused by soil-borne fungus, Rhizoctonia solani. This study was undertaken to assess antifungal potential of Carthamus oxycantha extracts and detection of likely antifungal components by GC-MS. In laboratory bioassays, antifungal activity of methanolic extracts of leaf, stem, root and inflorescence of C. oxycantha was checked using a range of concentrations from 1.562 to 200 mg mL-1 . The leaf extract significantly suppressed fungal growth. Methanolic leaf extract was subjected to GC-MS analysis. A total of 95 compounds were found present in this extract. Predominant compound was D-ribofuranose, 5-deoxy-5-(methylsulfinyl)-1,2,3-tris-O-(trimethylsilyl)- (13.312%) followed by benzoic acid, 4-hydroxy-3- methoxy-, methyl ester (11.888%), bis(2-ethylhexyl) phthalate (9.842%), 4-hydroxy-2,2',4',6'-tetrachlorobiphenyl, trimethylsilyl ether (6.837%) and pentanedioic acid (4.926%). Besides, a number of free fatty acids and fatty acid methyl esters with known antifungal potential were also identified as minor compounds.