Extraction, estimation and comparison of proteins and carbohydrates from different parts of Costus speciosus and a brief study on its phytochemicals content

摘要

Materials and methods: Extraction was carried out using 70% methanol for protein estimation while water extraction was done for carbohydrate quantification. Alcohol Test, Ring Test (Heller’s Test)[ Qualitative tests], (Lowry’s Method) and Modified Lowry’s Method[ Quantitative tests] were performed to detect and estimate the total proteins while Fehling’s Test, Benedict’s Test, Molisch’s Test [Qualitative tests] and Anthrone Method [Quantitative tests] were performed to detect and estimate the total carbohydrate content in different parts of the plant viz., leaves, stems and rhizome. Photochemical analysis for components like Saponin, Tannin, Steroids, Glycoside, Phytosterol and Flavanoid were done following previously described standard protocols.