Analysis of Sibling Pair Relationships of Balineses Indonesia,Using 12 STR Loci for Human Identification Process

摘要

Use of STR locus in studying the relations between siblings for the need of ethnic genetic database in Indonesia for identifying disaster victims, criminals and Probation Test (paternity test) is not well established compared to other countries. Targeted outcomes are STR typing and profiling of the human genome for individual identification and kin relations. The aim of this study was to analyse kin relationships among siblings by assessing and determining a better STR locus and the level of allelic distribution among full siblings in Balinese people of Indonesia. From a collection of 10 Balinese families, we took a sample of 4 people for each family consisting of the father, mother, and two children. Kin relationships were analysed by using 13 STR loci technique (CSF1PO, F13B, FES, TH01, TPOX, VWA, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11). Through the use of 13 autosomal STR loci, this study provides evidence that the strength of sharing 2 alleles can be used as a reference in that siblings have high kinship relations. Based on the results of this study, STR loci D8S1179 and S21S11 are recommended when typing the Balinese.