Benefits to Converting Old HPLC Methods to Optimized UPLC Methods

摘要

When considering a switch from traditional High-Performance Liquid Chromatography (HPLC) to Ultra-High Performance Liquid Chromatography (UPLC), there are several important factors to consider, such as how frequently is the test performed, how many samples are tested, what is the existing method, and what are the costs associated with testing. The most important consideration to most companies is going to be the impact of the change. Because the UPLC operates on the same principles as HPLC, the impact on analysts is minimal. The software and equipment operation are slightly different between the two; however, most of the skill and expertise directly transfers from HPLC to UPLC. Also, the regulatory impact is minimal as the FDA allows for changes that are “equivalent to or better than” existing test procedures and fall into the category of annual reportable changes.