INFLUENCE OF CALCIUM SOURCES ONMINERALIZATION OF CALCIUM CARBONATE BY PAENIBACILLUS FAVISPORUS U3

摘要

In this study, the effect of different calcium sources on calcium carbonate deposition by the ure-olytic Paenibacillus favisporus U3 was investigated. Calcium lactate, calcium nitrate, and calcium chlo-ride were used as organic and inorganic calcium sources. In addition, the activity of urease and car-bonic anhydrase enzymes required for calcium car-bonate mineralization by U3 was determined. Re-sults showed that the highest amount of CaCO was reached at 1000mM CaCl concentration. Enzyme activity studies proved that U3 contains urease and carbonic anhydrase enzymes. Also, calcite and va-terite forms of calcium carbonate were produced by the U3 strain. The calcite and vaterite crystals of cal-cium carbonate were verified by XRD. XRF. CRS. EPMA,and FESEM-EDX.Lastly,P.favisporus U3 can precipitate large amounts of calcium carbonate by tolerating high CaCl, concentration, demonstrat-ing its potential for use in soils with high calcium chloride concentrations.