ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF SECONDARY METABOLITE PRODUCED BY ENDOPHYTIC FUNGI ISOLATED FROM LANNEA COROMANDELICA (HOUTT.) MERR

摘要

Lannea coromandelica (Houtt.) Merr. which is known as Kayu Jawa in Bugis community, Indonesia has been widely used empirically for the treatment of wounds. Ethanol extract from its stem bark is known to have antibacterial, anti-inflammation, and antioxidant activity. The purpose of this study were to isolate, characterize, and determine the antioxidant and antimicrobial activity of the endophytic fungi from the roots of this plant. In this paper, we reported antioxidant and antimicrobial activity of the isolated endophytic fungi from Lannea coromandelica (Houtt.) Merr. and the isolation of the bioactive compound known as citrinin. The structure of citrinin was determined by extensive analysis of the spectroscopic data ( 1 H-NMR, 13 C-NMR, 2D NMR, and LC-MS) and comparison to those of the previous report. The antioxidant activity showed that the isolated compound has moderate activity with AAI value of 0.671 and IC 50 145.9 ppm while the antimicrobial activity demonstrated that the isolated compound was not active against Escherichia coli ATCC 25922, Salmonella typhi ATCC 14028, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853 (MIC: 1000 μg/ml).