A high-throughput BIA-MPA method for the simultaneous determination of amiloride and furosemide

摘要

In this work, a simple and fast batch injection analysis system with multiple pulse amperometric detection (BIA-MPA) was developed for the simultaneous determination of amiloride (AMD) and furosemide (FMD). A sample plug (150 ??L) was directly injected into the unmodified boron-doped diamond (BDD) electrode immersed in 0.1 mol La?’1 borate buffer (pH = 10). Simple and fast sample pre-treatment steps were required (dissolution and dilution). The analytical characteristics of the proposed method include good stability (RSD 1.1%; n = 20), low detection limits (0.13 and 0.94 mg La?’1 for AMD and FMD, respectively), high throughput (72 injections per h) and minimal waste production (a??150 ??L by analysis). The results obtained with the BIA-MPA method were compared to those obtained by HPLC; similar results were obtained (at a 95% confidence level).