Chromium (VI) tolerance and bioaccumulation by Candida tropicalis isolated from textile wastewater

摘要

In the present study a yeast strain isolated from industrial wastewater, identified as Candida tropicalis, showed chromium (Cr) tolerance level up to 5?mM. Yeast grown in minimal salt medium containing Cr (VI) ions for 48?h and crude enzyme extracts were tested for chromate reductase activity. Optimum temperature and pH of chromate reductase were 30?°C and pH of 7. The enzyme activity was greatly enhanced in the presence of divalent metal cations. Total protein profile revealed some protein bands were present in hexavalent chromium [Cr (VI)] treated samples but were absent in non-treated samples, especially low molecular-weight protein bands in the mass range of