NOVEL SYNTHESIS OF 4E,10E-4-(2-PHENYLDIAZENYL)-N- ((1H-INDOLE-3-YL)METHYLENE)BENZENAMINE AND ITS APPLICATION FLUORESCENT CHEMOSENSOR FOR Ag+ ION, CYTOTOXICITY ACTIVITY AND DFT STUDIES

摘要

A Schiff base of 4E,10E-4-(2-phenyldiazenyl)-N-((1H-indole-3-yl)methylene)benzenamine (PQ) was synthesized and confirmed by FT-IR, 1H & 13C NMR spectroscopy.Fluorescence responses on photo fluorescence spectroscopy to different metal ions have been studied.The sensor PQ exhibited highly selective for Ag+ ion with turn-on chemosensors confirmed by emission intensity at 364 nm while another fluorescence emission intensity at 522 nm.The stoichiometric complexes PQ+Ag+ behavior indicated that 1:1 ratio with association constant value of detecting as was calculated to be 2.33×106 M-1.The concentration of EDTA solution 0.5 equiv added with PQ+Ag+ solution diminished fluorescence intensity at 522 nm.It takes a long time to inhibit the cytotoxicity effect of HeLa cells treated with PQ of differing concentration results.The Gaussian output value of the energy gap value of the HOMOLUMO was found as ΔE=2.85 eV.