The aim of this study was to estimate the diversity and prevalence of both groups ofBrucella canis1 and 2 with and without deletion respectively in different areas of Argentina. A total of 104 bacterial cultures were typed asB. canisstrains using the classical biotyping method. Two PCR assays were performed to confirm that all isolates wereB. canisand notBrucella suis. The differentiation between groups 1 and 2 was achieved using another PCR assay and the diversity ofB. canisisolates was assessed with four MLVA_16 markers. All strains belonged to Group 2. Bruce 09 marker (MLVA_16 assay) showed the greatest diversity. Only Group 2 ofB. caniswas identified among the strains evaluated. The markers chosen from the MLVA_16 allowed us to detect genetic diversity among the strains ofB. canisstudied.
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