Isolation, Identification, and Preserval of Filamentous Fungi Found at Soils of Viotá, Cundinamarca

摘要

Objectives: This study focuses on the isolation, identification, and conservation of strains of filamentous fungi recovered from soils of Viotá, Cundinamarca; and the subsequent conformation of a strain collection. Methods: An adaptation of the sampling method proposed by Arias and Pi?eros was carried out. Identification was carried out by macroscopic, microscopic, and DNA sequencing. Conservation techniques were evaluated based on the index of effectiveness of the behavior of viability, purity, and morphological stability. An ANOVA TUKEY test was performed using both an alpha value of α = 0.05 with 95% confidence to assess significant differences.Results: Of the 125 samples analyzed, 165 isolates were recovered, and nine species were identified: Trichoderma spirale 15.53%; Trichoderma ovalisporum 13.66%; Hypocrea nigricans 13.04%; Penicillium implicatum 13.04%; Penicillium citrinum 11.80%; Fusarium incarnatum 11.18%; Fusarium verticiloides 9.94%; Cunninghamella elegans 6.83%, and Aspergillus cf. versicolor 4.97% that had the lowest number of isolates. Aspergillus, Penicillium, Trichoderma, Fusarium, and Cunninghamela are among the most common genera isolated soils. The most efficient conservation method was cryopreservation, with an efficiency rate of 100%. However, although the cultivation technique had an efficiency percentage of 70%, it is considered that it can be used in collections with limited financial support or as a backup preservation method. ángel, in 2006 supports the use of serial cultivation for periods of time such as those handled in this study (15 days). Application/Improvements: The results obtained will contribute to the knowledge of the diversity of this group and its biotic potentials; for the generation of strategies that promote the development of this region.