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Activity of polyphenoloxidase and peroxidase in non-dormant potato tubers treated with sprout suppressors

机译:用萌芽抑制器处理的非休眠马铃薯块茎中多酚氧化酶和过氧化物酶的活性

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摘要

One of the major problems affecting potatoes quality on stored is the susceptibility to enzymatic browning, which occurs mainly after wounds during the harvest, at postharvest handling and by aging, leading to the development of dark color compounds. Enzymatic browning is associated with the action of polyphenoloxidases (PPO) and peroxidases (POD), which use phenolic compounds and oxygen as substrates. The products of these enzymes cause undesirable changes of color and flavor of processed potato products. The present study aimed to evaluate some kinetic properties based to optimum pH and reaction temperature, and to determine changes on activity of potato tubers PPO and POD treated with sprouting suppressors compounds menthol and eugenol. For each treatment, samples were collected in six different periods, before application (day 0) and 10, 20, 30, 40, and 50 days after the treatments to determine the influence of sprout inhibitors on the activity of PPO and POD. Changes in pH and temperature affected drastically the activity of both enzymes. The use of eugenol and menthol as sprouting suppressors decreased enzymatic activity on the treated tubers compared to control with an inverse relationship between enzymes activity and content of phenolic compounds.
机译:影响储存的土豆质量的主要问题之一是酶促褐变的易感性,这主要发生在收获过程中伤口后,在采后处理和老化,导致深色化合物的发育。酶促褐变与多聚氧化酶(PPO)和过氧化物酶(POD)的作用有关,其使用酚类化合物和氧作为基材。这些酶的产品导致加工马铃薯产品的颜色和风味的不希望变化。本研究旨在评估基于最佳pH和反应温度的一些动力学性质,并确定用萌芽抑制剂化合物和丁烯醇处理马铃薯块茎PPO和豆荚活性的变化。对于每种处理,在治疗后六个不同的时期,在治疗后(第0天)和10,20,30,40和50天之前收集样品以确定萌芽抑制剂对PPO和豆荚活性的影响。 pH和温度的变化大大影响了两种酶的活性。与酚类化合物之间的反比关系进行控制相比,使用丁醇和薄荷醇作为萌发抑制剂的用途降低了处理过的块茎上的酶活性。
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