A mitochondria-targeted near-infrared probe for colorimetric and ratiometric fluorescence detection of hypochlorite in living cells

摘要

In this study, we report a near-infrared (NIR) fluorescent probe ( CMBI ) for the detection of hypochlorite (ClO ~(?) ), which displays colorimetric and ratiometric fluorescence dual responses towards ClO ~(?) . The probe can detect ClO ~(?) with high selectivity, fast response (within 90 s) as well as low detection limit (33 nM). An oxidation reaction was proposed for the sensing mechanism, which was confirmed by ~(1) H NMR and HR-MS spectra. Fluorescence co-localization studies demonstrated that CMBI was a specific mitochondria-targeted fluorescent probe for ClO ~(?) with excellent cell membrane permeability. Furthermore, confocal fluorescence images of HeLa cell indicated that CMBI could be used for monitoring intracellular ClO ~(?) in living cells by ratiometric fluorescence imaging.