Intrathymic inoculation of donor liver specific antigen alleviates rejection of liver allotransplantation

摘要

Use and effects of liver specific antigen in orthotopic b%5D=abstract&ck%5b%5D=keyword'>b>liver transplantations were researched in this study. Group I:syngeneic control (Wistar-to-Wistar); Group II:acute rejection (SD-to-Wistar); Group III: Thymic inoculation of SD rat LSA day 7 before transplantation. The observation of common situation and survival time, rejection grades, b%5D=abstract&ck%5b%5D=keyword'>b>NF-кb activity of splenocytes and IL-2mRNA expression of grafted liver were used to analyze acute rejection severity and immune state of animals in different groups. The common situation of group I was very well after transplantation and no signs of rejection were found. Recipients of group II lost body weight progressively. All dead within day 9 to day 13 posttransplantation; median survival time was 10.7±0.51 days. It was an optimal acute rejection control. As for group III, 5 out of 6 recipients survived for a long time and common situation was remarkably better than that of group II. Its rejection grades were significantly lower than that of group II(Pb>NF-кb activity was only detected in group I at day 5 and day 7 after transplantation, whereas high activity of b%5D=abstract&ck%5b%5D=keyword'>b>NF-кb was detected at all time points in groupII and the low b%5D=abstract&ck%5b%5D=keyword'>b>NF-кb activity detected in group III was significantly lower than that of group II (Pb>liver transplantation. We report here for the first time that intrathymic inoculation of LSA can alleviate the rejection of liver allotransplantation; and that grafts can survive for a long time thereby, thus leading to a novel way to achieve b%5D=abstract&ck%5b%5D=keyword'>b>liver transplantation b%5D=abstract&ck%5b%5D=keyword'>b>immunotolerance.