Pharmacological evaluation and mechanistic study of compound Xishu Granule in hepatocellular carcinoma

摘要

ObjectiveIn this study, we used HepG2 human hepatocellular carcinoma cells to study the effects of Compound Xishu Granule (CXG) on cell proliferation, apoptosis, and the cell cyclein?vitro. We also used a xenograft tumor model to study the anti-tumor effects of CXG and related mechanismsin?vivo.MethodsThe effect of CXG on cell viability was measured using Cell Counting Kit-8 and a colony formation assay. The effect of CXG on apoptosis and the cell cycle was analyzed using flow cytometry. Thein?vivoanti-tumor effect of CXG was assessed by measuring the volume change in xenograft tumors after drug administration. The CXG anti-tumor mechanism was studied using western blotting assay to detect cell cycle and apoptotic associated proteins.ResultsCXG suppressed HepG2 cell proliferation in a time- and dose-dependent mannerin?vitro. Colony formation experiments showed that CXG administration for 24?h significantly reduced HepG2 cell formations (P?