Genomic characterization of a pectinolytic isolate of Serratia oryzae isolated from lake water

摘要

Only one isolate of Serratia oryzae, the type strain J11-6T has been characterized up to now. Thisstrain was found in the endophytic bacterial flora of rice. As part of an ongoing investigation intopectinolytic bacteria present in lake water in France, a few Serratia strains were isolated, includingS32 and J9 identified as new strains of S. oryzae. The genome of strain S32 consists of a circularchromosome of 4,810,389 bp that contains 4,584 protein-coding genes. The genome of S32, as wellas those of the type strain J11-6T, contains several genes involved in pectin degradation and in theintracellular assimilation of pectin oligomers. The specific detection of enzyme activities confirmedthat strain S32 secretes functional pectinases and that it also produces extracellular cellulase andprotease activities. The ability to produce plant cell wall degrading enzymes shows that S. oryzaeshares characteristics of plant associated bacteria, including phytopathogens.