Roy et al. (2018. J. Cell. Biol. https://doi.org/10.1083/jcb.201709121) describe an ingenious single-cell assay system, in situ analysis of protein interactions at DNA replication forks (SIRF), for the quantitative analysis of protein interactions with nascent DNA at active and stalled replication forks. The sensitive and accurate SIRF methodology is suitable for multiparameter measurements in cell populations.
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机译：Roy等人。 （2018. J. Cell.Biol。HTTPS://Doi.org/10.1083/JCB.201709121）描述了一种巧妙的单细胞测定系统，原位分析DNA复制叉（SIRF）的蛋白质相互作用，用于定量分析在活性和停滞复制叉上与新生DNA的蛋白质相互作用。敏感和准确的SIRF方法适用于细胞群体中的多级测量。
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