In-Vitro Sorbent-Mediated Removal of Edoxaban from Human Plasma and Albumin Solution

摘要

Background and ObjectiveBased on previous experience of sorbent-mediated ticagrelor, dabigatran, and radiocontrast agent removal, we set out in this study to test the effect of two sorbents on the removal of edoxaban, a factor Xa antagonist direct oral anticoagulant.MethodsWe circulated 100?mL of edoxaban solution during six first-pass cycles through 40-mL sorbent columns (containing either CytoSorb in three passes or Porapak Q 50–80 mesh in the remaining three passes) during experiments using human plasma and 4% bovine serum albumin solution as drug vehicles. Drug concentration was measured by liquid chromatography-tandem mass spectrometry.ResultsEdoxaban concentration in two experiments performed with human plasma dropped from 276.8 to 2.7?ng/mL and undetectable concentrations, respectively, with CytoSorb or Porapak Q 50–80 mesh ( p =?0.0031). The average edoxaban concentration decreased from 407?ng/mL?±?216?ng/mL to 3.3?ng/mL?±?7?ng/mL ( p =?0.017), for a removal rate of 99% across all six samples of human plasma (two samples) and bovine serum albumin solution (four samples). In four out of the six adsorbed samples, the drug concentrations were undetectable.ConclusionSorbent-mediated technology may represent a viable pathway for edoxaban removal from human plasma or albumin solution.