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Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery

机译:无标记和等离的串联质量标签(TMT)多路复用的两种对比水稻品种的定量蛋白质组学数据,暴露于干旱胁迫和恢复

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Two rice cultivars, IAC1131 (drought tolerant) and Nipponbare (drought sensitive), with contrasting genetic backgrounds and levels of tolerance to drought, were analysed using both label-free and tandem mass tags (TMTs) quantitative proteomics approaches, aiming to elucidate the mechanisms of drought tolerance. Four-week-old seedlings of both cultivars were grown in large soil volumes in the glasshouse under controlled conditions and then exposed to moderate and extreme drought for 7 days, followed by 3 days of re-watering period. Mature leaves were harvested from plants of each treatment for protein extraction and subsequent complementary shotgun proteomic analyses. The data from this study are related to the research article “Quantitative proteomic analysis of two different rice varieties reveals that drought tolerance is correlated with reduced abundance of photosynthetic machinery and increased abundance of ClpD1 protease” (Wu et al., 2016) [1].
机译:使用标签和串联质量标签(TMTS)定量蛋白质组学方法,分析了两种稻米品种,具有对比的遗传背景和对干旱耐受性水平的遗传背景和对干旱水平的耐受性。旨在阐明机制干旱耐受性。在受控条件下玻璃馆的大型土壤体积生长了四周的两周幼苗,然后暴露于温和和极端的干旱7天,然后进行3天的再浇水。从每种处理的植物收获成熟的叶子,用于蛋白质提取和随后的互补霰弹枪蛋白质组学分析。本研究的数据与研究制品相关“两种不同水稻的定量蛋白质组学分析显示,耐旱耐受性与降低的光合作机和CLPD1蛋白酶增加的丰富量相关”相关性(Wu等,2016)[1] 。

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