From anaerobes to aerointolerant prokaryotes

摘要

An increasing number of scientists are turning to the microbiota to understand and/or explain the origin of various human metabolic or inflammatory diseases. Oxygen-intolerant bacteria represent the major population of the human intestinal microbiota. Their isolation is often difficult or even fastidious. The number of studies showing their beneficial role in human health is growing exponentially.Faecalibacterium prausnitziiandAkkermansia muciniphilaare abundantly represented in healthy intestinal microbiota and their imbalance is positively correlated with inflammatory diseases and metabolic disorders (obesity, diabetes, cancers). Their use as probiotics presents very promising results in restoring the balance of microbial flora but also in the treatment of certain pathological conditions. The Christensenellaceae family has recently emerged as a hereditary taxon and studies have shown that its abundance is positively correlated with leanness and controls obesity in recipient mice. Here, we report the different culture strategies and techniques used for their isolation; the role of antioxidants in the survival of these oxygen-sensitive species in clinical sample and their maintenance in culture isolates.