Effects of Hydroalcoholic Leaf Extract of Avicennia marina on Apoptotic, Inflammatory, Oxidative Stress, and Lipid Peroxidation Indices and Liver Histology of Type 1 Diabetic Rats

摘要

Background: Avicennia marina (A. marina) has been traditionally used in the treatment of various diseases due to its antiinflammatoryand antioxidant properties.Objectives: The study aimed to determine the effect of hydroalcoholic leaf extract of A.marinaon apoptotic, inflammatory, oxidativestress, and lipid peroxidation indices and liver histology of type 1 diabetic rats.Methods: In this laboratory research, a total of 40 male Wistar rats were randomly divided into five groups including the controlgroup, type 1 diabetes mellitus (DM) group, diabetic group receiving 50 mg/kg of hydroalcoholic extract of A. marina, diabeticgroup receiving 100 mg/kg of A. marina, and the diabetic group receiving 200 mg/kg of A. marina. The control and DM groups wereintraperitoneally administered with 0.5 mL of saline solution as the solvent for 30 days. The experimental groups were intraperitoneallyadministered with 0.5 mL of hydroalcoholic extract at doses of 50, 100, and 200 mg/kg for 30 days, respectively. Diabetesmellitus was induced by an intraperitoneal injection of 120 mg/kg of alloxan monohydrate. At the end of treatment, we measuredthe serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6. Also, we assessed the levels of BCL2-associated Xprotein (Bax), B-cell lymphoma 2 (Bcl-2), caspase-9, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), malondialdehyde(MDA), and 8-hydroxy-2’-deoxyguanosine (HOdG-8) using ELISA in liver tissues. Histological changes in the liver werealso investigated.Results: The levels of Bcl-2, SOD, CAT, and GPx were significantly higher in the experimental groups receiving 100 and 200 mg/kg ofA. marina in a dose-dependent manner than in the DM group. The levels of Bax, caspase-9, MDA, and HOdG-8 were significantly lowerin the experimental groups in a dose-dependent manner. Also, the serum levels of TNF-α, IL-1β, and IL-6 were significantly lowerin these groups in a dose-dependent manner (P 0.05). However, the differences were not significant in the experimental groupreceiving 50 mg/kg of A. marina (P 0.05). The results showed that A. marina extract improved the histological changes in the liverof diabetic rats in a dose-dependent manner.Conclusions: The administration of the hydroalcoholic extract of A. marina can improve liver tissue damage and reduce diabetesrelatedliver complications by decreasing apoptosis, inflammation, oxidative stress, and lipid peroxidation.