Plantlet Reproduction of King White Mulberry ( Morus macroura Miq) via Direct Organogenesis

摘要

In vitro propagation is apromising method in regeneration instead of difficult-to-producedplants through seeds or stem cuttings (traditional propagation methods). The starting explants were bothshoot tips and nodal cuttings explants taken from mature trees of king white mulberry (Morus macroura miq).Explants were cultured on Murashige and Skoog (MS) medium supplemented with both BA and Kin in variousconcentrations (1.0, 2.0, 4.0 and 6.0 mg/l) for each in a combination with IBA (0.1 mg/l). A significant inductionof direct organogensis with notable appearance of callus were observed at the high cytokinins concentrations.Nodal explants were more superior than shoot tips in proliferation producing more number of shoots. Resultsindicated that BA was the most suitable source of cytokinins for shoot multiplication at various comparablerates with Kin. Rooting formation was performed on MS medium amended with 2.0 mg/l of IBA plus 1.0 g/lactivated charcoal, which was sufficient to encourage the capability of roots formation. The acclimatized plantswith 75% survival rate were shifted to the field conditions with notable success.