Induction of Immune Responses against Glycosphingolipid Antigens: Comparison of Antibody Responses in Mice Immunized with Antigen Associated with Liposomes Prepared from Various Phospholipids

摘要

References(16) Cited-By(4) The immune responses of mice against glycosphingolipid (GSL) antigens and the effect of the phospholipid composition of liposomes on the immunogenicity in mice of liposome-associated GSL antigens were examined. The immunization with GSL antigen alone was unable to induce any detectable anti-GSL antibody responses. On the other hand, the immune responses against GSL antigens were detected after immunization with liposomes composed of dipalmitoylphosphatidylcholine (DPPC) (0.5 μmol), cholesterol (Chol) (0.5 μmol), Salmonella minnesota R595 lipopolysaccharides (LPS) (10 μg) and GSL (0.05 μmol) (DPPC-liposome). However, the administration with liposome composed of dimyristoylphosphatidylcholine (DMPC) (0.5 μmol), Chol (0.5 μmol), S. minnesota R595 LPS (10 μg) and GSL (0.05 μmol) and with liposomes composed of distearylphosphatidylcholine (DSPC) (0.5 μmol), Chol (0.5 μmol), and S. minnesota R595 LPS (10 μg) and GSL (0.05 μmol) was ineffective for the induction of the immune responses against GSL antigens. These results suggest that DPPC-liposome would serve effectively as a delivery vehicle for inducing immune responses against GSL antigen.