Simultaneous determination of carbamazepine, phenytoin and phenobarbital in dried blood spots by high performance liquid chromatography

摘要

Carbamazepine, phenobarbital and phenytoin were determined in dried blood spots (DBS) by high performance liquid chromatography, after extraction of 8 mm DBS using a mixture of acetonitrile and methanol. Analytes were separated by reversed-phase chromatography, with a run time of 17 minutes. Intra-assay and inter-assay precisions were in the 5.3 to 8.4% and 3.3 to 5.2% ranges, respectively. Accuracy was in the 98.8 to 104.3% range. The method had sensitivity to detect all analytes at levels below minimum therapeutic concentrations. The analytes were stable at 4 ?oC and room temperature for up to 12 days and at 45 ?oC for 9 days. The method was applied to 14 paired clinical samples of blood serum and DBS.