The aim of the study was to evaluate the yield and grain quality (1000 kernel weight, test weight, crude protein, starch, β-glucans, total phenolic content, antiradical scavenging activity and α-tocopherol content) variation of three hulless spring barley (Hordeum vulgare L.) genotypes under different levels of N, in relation to weather characteristics. The field experiments were carried out at the State Stende Cereal Breeding Institute. Three hulless barley genotypes ‘Kornelija’; ‘ST 1165’; ST 1185’ were studied during two years (2011 and 2012) using three levels of nitrogen (N80; N80+40; N80+80) by split application at the end of the tillering stage. The effects of genotype, N treatment and genotype by N treatment interaction contribute the variation in yield and grain quality traits for hulless barley genotypes. There were considerable differences between crop years in response of hulless genotypes to top-dressing N, with significantly (P -1. Hulless line ‘1185’ gave the highest grain yield, starch, α-tocopherol content and radical scavenging activity, but variety ‘Kornelija’ formed significantly higher TKW, crude protein and β-glucans content.
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