EVALUATION OF GENETIC DISTANCES CORRELATIONS AMONG SUGAR BEET GENOTYPES (BETA VULGARIS L.)

摘要

A search and a combination of different statistical methods for determiningthe similarity and difference between genotypes is a hot topic for breeding. In theformation of adaptive potential and appropriate protective mechanisms for theimplementation of the adaptive potential of sugar beet, terpene compounds andsaponins are involved.To determine polymorphism based on DNA of sugar beet materials underinvestigation, RAPD analysis was performed. A cluster analysis of the hybridsaffinity was carried out in terms of the qualitative biochemical state of the systemand DNA polymorphism. As a result, it was found that hybrids that demonstratedhigh activity of the secondary metabolism and, consequently, showed differentability to form a high adaptive potential in the conditions of long-term cultivationin vitro, formed separate clusters. With the aid of RAPD markers, the authorsalso determined two clusters that were formed on the basis of genotype affinity.In order to determine the correlations between genetic distances obtained byRAPD markers and terpene compounds, the Mantel test (linear correlation forPearson) was performed. The values of coefficients r = 0.517 were obtained at α= 0.05. However, according to the data interpretation, the calculated value of p(0.088) was higher than the significance level of α = 0.05, which indicates theabsence of correlations between the matrices under investigation. Thus, therevealed genotypic features of the secondary metabolism and genetic distancesare additional characteristics for evaluation of the ecological plasticity of sugarbeet plants, which is important in the breeding process.