Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm

摘要

Fertilisation in marine invertebrates, such as the sea urchin, occurs during broadcast spawning events in which males and females of co-localised species ejaculate sperm and spawn eggs synchronously. During these events, spermatozoa have to find and fertilise conspecific eggs in the midst of all the other ones, which is a remarkable navigation and mating choice achievement. Sperm cells do this by navigating towards the source of species-specific peptides released by the egg, steered by spatial and temporally orchestrated peaks in intracellular calcium concentration that trigger sudden reorientations. How these calcium spikes are regulated and timed remains elusive. Different calcium channels have been implicated by indirect experimental evidence giving rise to a complex network of putative interacting components. We gained insight into the structure and function of this network by modelling it as a set of candidate modules that could be studied separately. By using this 'divide and conquer' approach to the complexity of the network, we could characterise the potential dynamics of each module and confront these dynamics with specific quantitative data. Our results indicate that the channel mediating calcium signals in sea urchin sperm is likely CatSper, a calcium channel necessary for human male fertility.