The role of intracellular interactions in the collective polarization of tissues and its interplay with cellular geometry

摘要

Planar cell polarity (PCP) is an indispensable and conserved pathway in morphogenesis. In spite of the advances in understanding the different modules of PCP, a comprehensive picture of the intracellular protein-protein interactions necessary for the emergence of long-range tissue polarity is still lacking. In order to address this question, we devised a generalized reaction-diffusion model, through which we investigated the role of cytoplasmic interactions in PCP pathways. The length scale of intracellular interactions is demonstrated to be crucial to the stability of the cytoplasmic segregation of membrane proteins in disordered tissues, as well as the capacity of polarization field for detecting the gradient and geometrical cues. Finally, three classes of mutants are investigated within the context of our model. Comparison with the in vivo observations allows us to infer the major contributions of cytoplasmic proteins to the emergence of tissue polarity, and make testable predictions regarding the cooperation of cytoplasmic and membrane proteins in the coordination of collective polarization.