The effect of Jiedu Huoxue decoction on rat model of experimental nonbacterial prostatitis via regulation of miRNAs

摘要

Context The underlying mechanisms of Jiedu Huoxue decoction (JDHXD) in treating chronic prostatitis have not been fully explored. Objective This study investigates the miRNAs as potential biomarkers and the effect of JDHXD on the rat model of experimental nonbacterial prostatitis. Materials and methods Fifty-four Sprague-Dawley male rats were randomly divided into normal control, model, JDHXD low dose (0.5?g/kg/day), medium dose (1?g/kg/day), high dose (2?g/kg/day) and western medicine (cernilton 0.094?g/kg/day) groups, and intragastrically administered once daily for 30?days. The control and model (upon successful establishment) groups received distilled water. Differential expression of miRNAs was analysed with high-throughput miRNA sequencing and validated with qRT-PCR and Northern blot. Prediction of specific target genes and functional enrichment analysis were performed with bioinformatics. Results LDsub50/sub test showed no sign of toxicity with maximum feasible dose 4?g/kg JDHXD. Compared with control, 495 miRNAs showed expression changes in CAP/CPPS rats, of which 211 were significantly different and 37 were prostatic-related. There were 181 differentially expressed miRNAs between the model and high dose JDHXD groups, of which 23 were identical with the control and model groups. Compared with control, miR-146a, miR-423 and miR-205 expression increased significantly in the model group, decreased dose-dependently in the JDHXD groups (p??0.05), and vice-versa for miR-96 (p??0.05). The effect of low dose JDHXD was comparable to cernilton (p??0.05). Discussion and conclusions Future studies may explore the contributions of the active components in JDHXD. The study design is generalisable. The effect can be repeatedly verified in clinical trials.