Green biosynthesis of silver nanoparticles using pomegranate peel and inhibitory effects of the nanoparticles on aflatoxin production

摘要

In this work, pomegranate peel has been used as a natural and safe method for biosynthesis of silvernanoparticles. Thesynthesis of silver nanoparticles was confirmed using UV spectroscopy, which showed a peak around a wavelength of 437nm. The morphology showed spherical and monodispersed nanoparticles with a size range between 5-50 nm. Usingscanning electron microscopy (SEM) and transmission electron microscopy (TEM), X-ray diffraction (XRD) experimentsrevealed their crystalline nature. Active functional groups in the synthesized silver nanoparticles were determined usingFourier transform infrared (FTIR) spectrometers contained four bands at 3281.21 cm-1, possibly indicating theparticipationof O–H functional group. The peak take place at 1,636.22 cm-1 may be pointed to C = N bending in the amidegroup or C = O stretching in carboxyl. Transfer in this peak (from 1,641 to 1,643 cm-1) shown the possible role of aminogroups or carboxyl in nanoparticle synthesis. The peaks at 431.95 and 421.28 cm-1 be related to AgNPs bonding with oxygenfrom hydroxyl groups which confirm the role of pomegranate peel as a reducing agent. Furthermore, we investigated effectsof these nanoparticles on aflatoxin B1 production by the fungus Aspergillus flavus, isolated from hazelnut. The results foundthat aflatoxin production in all A. flavus isolates decreased with an increase in the concentration of silver nanoparticles.Maximum suppression of aflatoxin production was recorded at a nanoparticle concentration of 150 ppm.