Assessing the effect of phytohormone on growth and germination of soybean (Glycine max (L.) Merr.) from cotyledonary node

摘要

Glycine max (L.) Merr. production is affected by a number of biotic and abiotic stresses. In order to overcome these challenges, development of transgenic plants, production of high-quality varieties and secondary metabolites, preservation of rare plants, and protection of germplasms is of prime importance. In this regard, proper regeneration system for plants growth is needed to overcome the dormancy and low germination issues by using appropriate growth hormones. Therefore, we analyzed the effect of various phytohormones 2, 4-dichlorophenoxyacetic acid (2, 4-D), 6-benzyladenine (BAP), Kinetin (KIN) and Indole-3-butryic acid (IBA) on Glycine max growth to investigate the influences of explants types. Results revealed that good callus is produced from cotyledonary node on 2, 4-D (4.0 mg L -1 ). The highest percentage of regenerated shoots (88%) was found on BAP+KIN (2.0+1.0 mg L -1 ) with maximum shoots number (3.00±0.09) and maximum length of shoot is (4.22±0.08) cm. The IBA (0.5 mg L -1 ) is excellent for root formation. Here, applied procedure used for further development of regeneration and transformation efficiency of Glycine max .