Estimation of genetic diversity and selection of high yielding genotypes in Phaseolus vulgaris (L.) germplasm

摘要

In present study 45 exotic Phaseolus vulgaris (L.) genotypes were assessed for assessment of genetic diversity through morphological & biochemical (SDS-PAGE) traits and selection of high yielding genotypes. One ways cluster dendrogram analysis of morphological traits segregated total genotypes to 2- lineages, lineages-1 and -2, separated at 80% similarity level. The lineages-1 further sub divided into 2 clusters i.e. cluster (C-I) and cluster (C-II), whereas lineage-2 consisted of one cluster (C-III). The high yielding genotypes were G3185 G (410g/plant) and G2785 (410g/plant) followed by G2613 (169g/plant) and G3185 (149g/plant). Biochemical characterization revealed that 11 reproducible polypeptide bands were observed in which 18.18% protein bands (band 2, 5) were monomorphic and 81.82% were polymorphic. Among polymorphic bands, band six showed outmost genetic diversity (17.7%) followed by band four and band eight showed 15.5% genetic diversity. Two way cluster dendrogram of biochemical characterization separated genotypes into two lineages L-1 and L-2. L-1 comprises of 20% genotypes with 50% genetic diversity, while L-2 comprisesed of 80% genotypes with 16.5% genetic diversity. Additional selection, multi locational trials for yield, and resistant to abiotic stress is significantly needed while, genotypes having similar banding pattern need additional characterization through morphological and 2-D electrophoresis in conjunctions with DNA based analysis.